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172 flow cytometry analyses  (Thermo Fisher)


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    Thermo Fisher 172 flow cytometry analyses
    172 Flow Cytometry Analyses, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+analyses/5'-Ethynyl-2'-deoxyuridine/10__1158_slash_1541___7786__mcr___25___1310-88-0-29
    Average 99 stars, based on 1 article reviews
    172 flow cytometry analyses - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Patterns and trajectories of peripheral inflammatory cytokines, immune tolerance, and lymphocyte differentiation predict transition from acute to chronic low back pain in a sex and age specific manner
    Article Snippet: Prior to thawing PBMCs, wells of a 96-well plate were pre-loaded with 50μl RPMI-10 containing nothing (Mock); 100ng PAM 3 CSK 4 (TLR1/2 ligand, Invivogen); 10ng lipopolysaccharide (LPS, TLR4 ligand, Invivogen); 100ng R848 (TLR7/8 ligand, Invivogen); high molecular weight poly I:C-lyovec (MDA5 ligand, Invivogen); ISD-lyovec (cGAS-STING activator, Invivogen); 500ng mouse anti-human CD40 antibody (αCD40, R&D systems MAB6321) with 2μg goat anti-mouse IgG antibody (Jackson ImmunoResearch, for crosslinking); or 200ng anti-CD3 antibody (BioLegend), 200ng Ultra-Leaf anti-CD28 antibody (BioLegend), with 1.6μg goat anti-mouse IgG (for crosslinking). .. After thawing and treating PBMCs with DNAse as described for ‘flow cytometry analyses’ above, cells were counted using a Countess II with trypan blue exclusion (Thermo-Fisher) and 5×10 4 viable PBMCs in 50μl RPMI-10 were added to wells containing each of the 8 conditions. ..

    Article Title: Presence of SopE and mode of infection result in increased Salmonella-containing vacuole damage and cytosolic release during host cell infection by Salmonella enterica.
    Article Snippet: A cc ep te d A rti cl e SCV integrity out with a Zeiss microscope (63x objective) and an environmental incubation chamber at an atmosphere of 5 % CO2. .. Quantitation by flow cytometry analyses HeLa cells were infected at a MOI of 1 to 150 for 25 min. At various time points from 1 to 16 h p.i., cells were detached with Biotase and fixed with 3 % PFA for subsequent flow cytometry analyses using the Attune NxT Cytometer (Thermo Fischer). ..

    Article Title: DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome
    Article Snippet: .. Two monoclonal antibodies were used for flow cytometry analyses: anti-CD11b (M1/70; Affymetrix eBioscience, 48-0112-82) and anti-F4/80 (BM8; BioLegend, 123109). .. Two monoclonal antibodies were used for flow cytometry analyses: anti-CD11b (M1/70; Affymetrix eBioscience, 48-0112-82) and anti-F4/80 (BM8; BioLegend, 123109).

    Article Title: Isogenic pairs of induced-pluripotent stem-derived endothelial cells identify DYRK1A/PPARG/EGR1 pathway is responsible for Down syndrome-associated pulmonary hypertension.
    Article Snippet: .. Immunofluorescence and flow cytometry analyses For immunocytochemical analysis, we fixed the cells with 4% paraformaldehyde for 10 min, and permeabilized it with a PBS solution containing 0.1% of Triton-X 100 for 10 min. After blocking with 5% FBS, we incubated the cells with anti-CD31 (1/30; 14- 0319-82, Thermo Fisher Scientific, Waltham, MA, USA) primary antibody at 4◦C overnight. .. Afterward, we incubated the cells with secondary Goat anti-Mouse Alexa Fluor 488 antibody (1/400; A11001, Thermo Fisher Scientific) for 60 min at room temperature, and we stained the nuclei with Hoechst 33 342 (H342, Dojindo Molecular Technologies, Kumamoto, Japan).

    Quantitation Assay:

    Article Title: Presence of SopE and mode of infection result in increased Salmonella-containing vacuole damage and cytosolic release during host cell infection by Salmonella enterica.
    Article Snippet: A cc ep te d A rti cl e SCV integrity out with a Zeiss microscope (63x objective) and an environmental incubation chamber at an atmosphere of 5 % CO2. .. Quantitation by flow cytometry analyses HeLa cells were infected at a MOI of 1 to 150 for 25 min. At various time points from 1 to 16 h p.i., cells were detached with Biotase and fixed with 3 % PFA for subsequent flow cytometry analyses using the Attune NxT Cytometer (Thermo Fischer). ..

    Infection:

    Article Title: Presence of SopE and mode of infection result in increased Salmonella-containing vacuole damage and cytosolic release during host cell infection by Salmonella enterica.
    Article Snippet: A cc ep te d A rti cl e SCV integrity out with a Zeiss microscope (63x objective) and an environmental incubation chamber at an atmosphere of 5 % CO2. .. Quantitation by flow cytometry analyses HeLa cells were infected at a MOI of 1 to 150 for 25 min. At various time points from 1 to 16 h p.i., cells were detached with Biotase and fixed with 3 % PFA for subsequent flow cytometry analyses using the Attune NxT Cytometer (Thermo Fischer). ..

    Cytometry:

    Article Title: Presence of SopE and mode of infection result in increased Salmonella-containing vacuole damage and cytosolic release during host cell infection by Salmonella enterica.
    Article Snippet: A cc ep te d A rti cl e SCV integrity out with a Zeiss microscope (63x objective) and an environmental incubation chamber at an atmosphere of 5 % CO2. .. Quantitation by flow cytometry analyses HeLa cells were infected at a MOI of 1 to 150 for 25 min. At various time points from 1 to 16 h p.i., cells were detached with Biotase and fixed with 3 % PFA for subsequent flow cytometry analyses using the Attune NxT Cytometer (Thermo Fischer). ..

    Immunofluorescence:

    Article Title: Isogenic pairs of induced-pluripotent stem-derived endothelial cells identify DYRK1A/PPARG/EGR1 pathway is responsible for Down syndrome-associated pulmonary hypertension.
    Article Snippet: .. Immunofluorescence and flow cytometry analyses For immunocytochemical analysis, we fixed the cells with 4% paraformaldehyde for 10 min, and permeabilized it with a PBS solution containing 0.1% of Triton-X 100 for 10 min. After blocking with 5% FBS, we incubated the cells with anti-CD31 (1/30; 14- 0319-82, Thermo Fisher Scientific, Waltham, MA, USA) primary antibody at 4◦C overnight. .. Afterward, we incubated the cells with secondary Goat anti-Mouse Alexa Fluor 488 antibody (1/400; A11001, Thermo Fisher Scientific) for 60 min at room temperature, and we stained the nuclei with Hoechst 33 342 (H342, Dojindo Molecular Technologies, Kumamoto, Japan).

    Blocking Assay:

    Article Title: Isogenic pairs of induced-pluripotent stem-derived endothelial cells identify DYRK1A/PPARG/EGR1 pathway is responsible for Down syndrome-associated pulmonary hypertension.
    Article Snippet: .. Immunofluorescence and flow cytometry analyses For immunocytochemical analysis, we fixed the cells with 4% paraformaldehyde for 10 min, and permeabilized it with a PBS solution containing 0.1% of Triton-X 100 for 10 min. After blocking with 5% FBS, we incubated the cells with anti-CD31 (1/30; 14- 0319-82, Thermo Fisher Scientific, Waltham, MA, USA) primary antibody at 4◦C overnight. .. Afterward, we incubated the cells with secondary Goat anti-Mouse Alexa Fluor 488 antibody (1/400; A11001, Thermo Fisher Scientific) for 60 min at room temperature, and we stained the nuclei with Hoechst 33 342 (H342, Dojindo Molecular Technologies, Kumamoto, Japan).

    Incubation:

    Article Title: Isogenic pairs of induced-pluripotent stem-derived endothelial cells identify DYRK1A/PPARG/EGR1 pathway is responsible for Down syndrome-associated pulmonary hypertension.
    Article Snippet: .. Immunofluorescence and flow cytometry analyses For immunocytochemical analysis, we fixed the cells with 4% paraformaldehyde for 10 min, and permeabilized it with a PBS solution containing 0.1% of Triton-X 100 for 10 min. After blocking with 5% FBS, we incubated the cells with anti-CD31 (1/30; 14- 0319-82, Thermo Fisher Scientific, Waltham, MA, USA) primary antibody at 4◦C overnight. .. Afterward, we incubated the cells with secondary Goat anti-Mouse Alexa Fluor 488 antibody (1/400; A11001, Thermo Fisher Scientific) for 60 min at room temperature, and we stained the nuclei with Hoechst 33 342 (H342, Dojindo Molecular Technologies, Kumamoto, Japan).



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    The ploidy levels assessments based on flow cytometry and chromosome counting of colchicine-treated and non-treated M. mongolica plantlets. Flow cytometry analysis of tetraploid ( A ) and diploid ( B ) plantlets; Chromosome counting slides of tetraploid ( C , 2n = 4x = 56) and diploid ( D , 2n = 2x = 28). Bar = 5㎛

    Journal: BMC Plant Biology

    Article Title: Induction and phenotype analysis of autotetraploids of Morus Mongolica

    doi: 10.1186/s12870-025-08058-5

    Figure Lengend Snippet: The ploidy levels assessments based on flow cytometry and chromosome counting of colchicine-treated and non-treated M. mongolica plantlets. Flow cytometry analysis of tetraploid ( A ) and diploid ( B ) plantlets; Chromosome counting slides of tetraploid ( C , 2n = 4x = 56) and diploid ( D , 2n = 2x = 28). Bar = 5㎛

    Article Snippet: The ploidy level after colchicine treatment was preliminarily determined by flow cytometry analysis CyFlow ® Ploidy Analyser (Sysmex Partec, Germany).

    Techniques: Flow Cytometry